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By immunohistochemistry, anti-Hu stains the nuclei of neurons.

To localize m RNA in brain tissue, one can use a fragment of DNA or RNA as a neuronal lineage marker, a hybridization probe that detects the presence of nucleotide sequences that are complementary to the sequence in the probe. Its application have been carried out in all different tissues, but particularly useful in neuroscience.

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It can also be a protein tag, as a partial protein, a protein or an epitope that discriminates between different cell types or different states of a common cell.

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The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells.