Elucidating isoniazid resistance using molecular
Isoniazid (INH) is front-line anti-tuberculosis (TB) drugs, which are usually prescribed to TB patients for a total period of 6 months.
Antituberculosis drug-induced hepatotoxicity (ATDH) is a serious adverse reaction of TB treatment.
First, the mycobacterial cell is surrounded by a specialized, highly hydrophobic cell wall that results in decreased permeability to many compounds (Fig.
to antimycobacterial drugs is the consequence of spontaneous mutations in genes that encode either the target of the drug, or enzymes that are involved in drug activation.
are bi-functional, heme-dependent enzymes that exhibit a strong catalatic activity comparable with that of monofunctional catalases and a broad-spectrum peroxidatic activity.
The proposed role of the enzyme is to protect bacteria from toxic molecules including hydroperoxides and hydroxyl radicals that are present in an aerobic environment.
The structure reveals new information about dimer assembly and provides information about the location of residues that may play a role in catalysis including candidates for protein-based radical formation.
which have vastly simplified and increased the speed of diagnosing drug-resistant TB, appear to suffer from variable sensitivity and specificity depending on the geographic region of the world in which they are employed.
CPs are generally homodimers or homotetramers with subunits of about 80 k Da.